COLORIMETRIC DETERMINATION OF PHOSPHORUS.

Colorimetric vs enzymatic determination of serum phosphorus.

Colorimetric Determination of Serum

In the past few years there has been renewed interest in the study of serum cholinesterase in various disease states, particularly those affecting the liver. Recently, a colorimetric method for the determination of this enzyme in the serum was reported by Ravin, Tsou, and Seligman (1). Their method, simple to perform, is as accurate as the gastrometric technique and more specific than the elect...

Enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine.

The performance of an enzymatic colorimetric method for the determination of inorganic phosphorus in serum and urine is described. Phosphate ions react with inosine in the presence of purine nucleoside phosphorylase to form hypoxanthine; this is oxidized by xanthine oxidase to uric acid with production of hydrogen peroxide. The latter is determined with the aid of the chromogen system peroxidas...

Colorimetric determination of blood arginase.

N EXTENSIVE LITERATURE Ofl arginase activity in tissues and tissue homogenates has accumulated in recent years. Roberts (1) has studied the arginase activity of liver, kidney, and epidermis of the Swiss mouse and of a transplantable squamous cell carcinoma. He found that liver had the greatest activity, followed by carcinoma, epidermis, and kidney. Sano (2) isolated an enzyme from rabbit intest...

Colorimetric determination of blood lipides.

Extraction-As the oxidation is essentially non-specific, the plasma extract must be as free of non-lipide reducing substances as possible. All solvents should be tested occasionally for reducing residues remaining after evaporation, and redistilled if necessary. Ether should be maintained peroxide-free. The determinations reported here followed a conventional alcohol-ether extraction and subseq...